Mg-incorporated micro/nano-topographical calcium silicate coatings with enhanced osteogenic properties and reduced inflammatory reactions

Abstract Orthopedic implant coatings with optimized surface topography and chemistry can achieve favorable osteogenesis inflammatory responses. In this work, to take advantage of micro/nano-topography nutrient element Mg, atmosphere plasma spray hydrothermal treatment were employed fabricate two kinds Mg-incorporated micro/nano-topographical calcium silicate 0.9 15.7 wt% Mg content (Mg1-CS Mg2-CS). MgSiO 3 microspheres composed nano-flakes formed on the CS coating surface. We investigated effects released ion protein adsorption behaviors bone mesenchymal stem cells (BMSCs) RAW264.7 macrophages. Compared coating, Mg2-CS had 1.8-fold increase in specific area, which favored serum BMSC adhesion. With higher 2+ release, Mg1-CS exerted greater effect enhancing fibronectin adsorption, integrin activation, osteogenic BMSCs. The gene expression profiles showed that could modulate macrophage polarization towards M2 phenotype showing effect. These results nanostructured Mg-containing promote responses mitigate reactions.